R4i Gold 3DS can run on Newest Nintendo 3DS V3.0.0-5 successfully – confimred by r4ids.cn on 7th. Dec,2011
As we know that Nintendo 3DS firmware updated to V3.0.0-5 . R4i Gold is the first R4i Card that work on 3DS V3.0.0-5 successfully , without any patch. more information please check here
Please note: As long as your R4i Gold 3DS card support 3DS V2.2.0-4, then the R4i Gold Card will support V3.0.0-5 automatically (You might need to do V2.2.0-4 patchupdating for R4i Gold 3DS card first in some case).